324715

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

• Synonyme(s) : Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
• Numéro CAS: 72561-05-8
• Numéro de classification (Commission des enzymes): 3.4.21.50 (BRENDA, IUBMB)
• Numéro MDL: MFCD02253020
• Code UNSPSC : 12352202

Propriétés

• Qualité: Proteomics Grade
• Niveau de qualité: 100
• Pureté: ≥90% (SDS-PAGE)
• Forme: lyophilized
• Activité spécifique: ≥200 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Avertissement

Toxicity: Harmful (C)

Définition de l'unité

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.

Forme physique

Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Reconstitute in 50 µl of distilled H₂O.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany