219365

Coagulation Factor V, Human Plasma

Coagulation Factor V, Human Plasma, CAS 9001-24-5, is a native coagulation factor V that is cleaved by thrombin to yield activated Factor Va that is 50-fold greater activity than its precursor form.

• Synonyme(s) : Coagulation Factor V, Human Plasma, Proaccelerin, Labile Factor, Ac-globulin, Ac-g
• Numéro CAS: 9001-24-5
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (SDS-PAGE)
• Forme: liquid
• Activité spécifique: ≥20 units/mg protein (clotting assay)
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Native coagulation factor V from human plasma. Thrombin cleaves Factor V to yield activated Factor Va, which exhibits 50-fold greater activity than the precursor molecule. Factor Va, Ca²⁺, and membrane phospholipids are cofactors that form a "prothrombinase" complex with Factor X, enabling its proteolytic activation of prothrombin to thrombin.

Native coagulation factor V from human plasma. Thrombin cleaves Factor V to yield activated Factor Va, which exhibits 50-fold greater activity than the precursor molecule. Factor Va, Ca²⁺, and membrane phospholipids are cofactors which form a "prothrombinase" complex with Factor Xa, enabling its proteolytic activation of prothrombin to thrombin.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 50% glycerol/H₂O (v/v).

Notes préparatoires

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Do not store below -20°C.

Remarque sur l'analyse

Clotting activity is determined by comparison to a standard curve produced using normal pooled human plasma

Autres remarques

Walker, F.J. 1992. J. Biol. Chem.267, 19896.
Monkovic, D.D., and Tracy, P.B. 1990. J. Biol. Chem.265, 17132.
Krishnaswamy, S., et al. 1989. J. Biol. Chem.264, 3160.
Lee, C.D., and Mann, K.G. 1989. Blood73, 185.
Krieg, U.C., et al. 1987. Biochemistry26, 103.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2