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Key Documents

203391

Sigma-Aldrich

(-)-Blebbistatin

≥98% (HPLC), solid, ATPase inhibitor, Calbiochem®

Synonyme(s) :

(-)-Blebbistatin

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About This Item

Formule empirique (notation de Hill):
C18H16N2O2
Numéro CAS:
856925-71-8
Poids moléculaire :
292.33
Numéro MDL:
MFCD08460907
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

product name

(-)-Blebbistatin, The active enantiomer of (±)-Blebbistatin that accounts for the inhibitory activity towards ATPase and myosin II-dependent cellular processes.

Niveau de qualité

100

Pureté

≥98% (HPLC)

Forme

solid

Puissance

2 μM IC50

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
protect from light

Couleur

yellow

Solubilité

methanol: 1.5 mg/mL
100% DMSO: 100 mg/mL
90% DMSO: 75 mg/mL

Conditions d'expédition

wet ice

Température de stockage

−20°C

InChI

1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3/t18-/m1/s1

Clé InChI

LZAXPYOBKSJSEX-GOSISDBHSA-N

Description générale

The active enantiomer of (±)-Blebbistatin (Cat. No. 203390) that accounts for the inhibitory activity towards ATPase (IC50 = ~2 µM) and myosin II-dependent cellular processes. Also reported to potently inhibit the actomyosin ATPase activities of expressed smooth muscle myosin IIA (SMA) and smooth muscle myosin IIB (SMB) smooth muscle myosin II heavy-chain isoforms (IC50 ~3 µM).
The active enantiomer of (±)-Blebbistatin (Cat. No. 203390) that accounts for the inhibitory activity toward ATPase (IC50 ~2 µM) and myosin II-dependent cellular processes. Also reported to potently inhibit the actomyosin ATPase activities of expressed smooth muscle myosin IIA (SMA) and smooth muscle myosin IIB (SMB) smooth muscle myosin II heavy-chain isoforms (IC50 ~3 µM).

Actions biochimiques/physiologiques

Cell permeable: no
Product does not compete with ATP.
Reversible: no

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month in 90% DMSO or methanol (-20°C). Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.

Autres remarques

Eddinger, T.J. et al. 2007. J. Pharmacol. Exp. Ther.320, 865.
Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Andrew D Doyle et al.
Developmental cell, 56(6), 826-841 (2021-03-12)
We describe a cellular contractile mechanism employed by fibroblasts and mesenchymal cancer cells to migrate in 3D collagen gels. During 3D spreading, fibroblasts strongly deform the matrix. They protrude, polarize, and initiate migration in the direction of highest extracellular matrix
Dennis J Yuan et al.
Biomaterials, 273, 120797-120797 (2021-04-21)
T cell activation is sensitive to the mechanical properties of an activating substrate. However, there are also contrasting results on how substrate stiffness affects T cell activation, including differences between T cells of mouse and human origin. Towards reconciling these
Takeshi Shimizu et al.
Journal of neurochemistry, 150(2), 158-172 (2018-12-28)
Oligodendrocytes (OLs) are myelinating cells of the central nervous system. Recent studies have shown that mechanical factors influence various cell properties. Mechanical stimulation can be transduced into intracellular biochemical signals through mechanosensors, such as integrin, p130Cas, talin and vinculin. However
Adam W Watson et al.
Cell reports, 35(13), 109293-109293 (2021-07-01)
While the immediate and transitory response of breast cancer cells to pathological stiffness in their native microenvironment has been well explored, it remains unclear how stiffness-induced phenotypes are maintained over time after cancer cell dissemination in vivo. Here, we show that
Takeshi Shimizu et al.
Glia, 65(2), 360-374 (2016-11-04)
Oligodendrocytes (OLs) are myelinating cells of the central nervous system. Recent studies have shown that mechanical factors influence various cell properties. Mechanical stimuli can be transduced into intracellular biochemical signals through mechanosensors and intracellular mechanotransducers, such as YAP. However, the
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