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Sigma-Aldrich

Magna ChIP® A - Chromatin Immunoprecipitation Kit

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About This Item

Conditionnement

kit of 22 assay(s)

Niveau de qualité

100

Fabricant/nom de marque

Magna ChIP®

Technique(s)

immunoprecipitation (IP): suitable

Description générale

Chromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

Application

Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A beads.

Caractéristiques et avantages

  • Faster:Magnetic protein A beads allow for the entire ChIP protocol to be done in as little as a day! All reagents to process your samples are included - you don′t have to spend valuable time making them.
  • Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions.

Conditionnement

Two boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Composants

  • Magnetic Protein A Beads
  • ChIP Dilution Buffer
  • Low Salt Wash Buffer
  • High Salt Wash Buffer
  • LiCl Wash Buffer
  • TE Buffer
  • Cell Lysis Buffer
  • Nuclear Lysis Buffer
  • ChIP Elution Buffer (w/o Proteinase K)
  • 10X Glycine
  • 10X PBS
  • Protease Inhibitor Cocktail II
  • Proteinase K
  • Spin Filters
  • Collection Tubes
  • Bind Reagent A
  • Wash Reagent B
  • Elution Reagent C

Stockage et stabilité

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.

Informations légales

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

FlameExclamation mark
GHS02,GHS07

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

55.4 °F

Point d'éclair (°C)

13 °C

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