17-283
Rac1 Activation Assay Kit
Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.
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About This Item
Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.32
Produits recommandés
Niveau de qualité
Espèces réactives
human, rat, mouse
Fabricant/nom de marque
Upstate®
Technique(s)
activity assay: suitable
affinity binding assay: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Informations sur le gène
human ... RAC1(5879)
Description générale
Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Ras-related C3 botulinum toxin substrate 1 (Rac1)-GTP from cell lysates and detected by a Rac1 specific monoclonal antibody. Rac1 belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). It is expressed in two major forms such as an inactive GDP-bound form and an active GTP-bound form. Rac1 is involved in influencing several physiological conditions by interacting with effectors that activate several signaling cascades. The RAC1 gene is located on the human chromosome at 7p22.1.
Application
Rac1 Activation Assay Kit has been used in the detection of active Ras-related C3 botulinum toxin substrate 1 (Rac1) in brain homogenates of mice and humans by immunoblotting.
Composants
Anti-Rac1, clone 23A8 (Cat.# 05-389)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
Qualité
Routinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac.
Liaison
Replaces: SGT425
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
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