14-383
Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg
Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.
Synonyme(s) :
Rho Assay Reagent, Rho Detection Reagent, Rhotekin RBD Assay Reagent
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About This Item
Code UNSPSC :
12352200
eCl@ss :
32160405
Nomenclature NACRES :
NA.84
Produits recommandés
Source biologique
mouse
Niveau de qualité
Fabricant/nom de marque
Upstate®
Technique(s)
affinity binding assay: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Informations sur le gène
mouse ... RTKN(20166)
Description générale
GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.
Product Source: Expressed in E. coli
Qualité
Routinely evaluated by precipitating GTP-Rho from 3T3/A31 lysates, subsequent detection by immunoblot analysis using Anti-RhoA, clone 55 (05-778).
Forme physique
650 µg of recombinant protein in 1 mL glutathione-agarose slurry containing 45mM TRIS, pH 7.5, 0.45% Triton X-100, 135mM NaCl, 4.5mM MgCl2, 0.9mM dithiothreitol, 0.09mM PMSF, 0.9 µg/mL aprotinin, 0.9 µg/mL leupeptin, and 10% glycerol.
Stockage et stabilité
Stable for 6 months at -70°C from date of receipt.
Autres remarques
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Determination of GTP loading on Rho.
X D Ren et al.
Methods in enzymology, 325, 264-272 (2000-10-19)
Progestogens regulate endothelial actin cytoskeleton and cell movement via the actin-binding protein moesin.
Xiao-Dong Fu, Marina Flamini, Angel Matias Sanchez, Lorenzo Goglia, Maria Silvia Giretti et al.
Molecular Human Reproduction null
Geranylgeranyl diphosphate depletion inhibits breast cancer cell migration.
Amel Dudakovic,Huaxiang Tong,Raymond J Hohl
Investigational New Drugs null
Minji Jo et al.
The Journal of biological chemistry, 277(14), 12479-12485 (2002-01-24)
Binding of the urokinase-type plasminogen activator (uPA) to its receptor activates diverse cell signaling pathways. How these signals are integrated so that cell physiology is altered remains unclear. In this study, we demonstrated that migration of MCF-7 breast cancer cells
Simvastatin reduces human atrial myofibroblast proliferation independently of cholesterol lowering via inhibition of RhoA
Porter, K. E., et al
Cardiovascular Research, 61, 745-755 (2004)
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