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1.52021

Supelco

Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 50 mm x 4.6 mm HPLC Column

Synonyme(s) :

Chromolith® HighResolution RP-18 endcapped 50-4.6 HPLC column

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About This Item

Code UNSPSC :
41115709
Nomenclature NACRES :
SB.52

product name

Chromolith® HighResolution RP-18 endcapped, L × I.D. 50 mm x 4.6 mm HPLC column

Matériaux

PEEK column

Niveau de qualité

Agence

suitable for USP L1

Gamme de produits

Chromolith®

Caractéristiques

endcapped
endcapped

Conditionnement

1 ea of

Ampleur du marquage

15% Carbon loading
18% Carbon loading

Paramètres

200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.

Technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)

Colonne, L × D.I.

5 cm × 4.6 mm

Matrice

highly porous
monolithic silica
monolithic silica

Groupe de la matrice active

C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase

Dimension de pores

1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity

operating pH range

2-7.5
2-7.5

Technique de séparation

reversed phase

Température de stockage

no temp limit

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Description générale

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping.

Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for very fast separations on any HPLC System and rapid method devlopment.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]

The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column).
[151467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: [UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns]

Remarque sur l'analyse

Pressure: 15 - 50 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 135000
Capacity factor (Anthracene): 3.2 - 4.2
Tailing (USP): 0.90 - 1.35 %
Selectivity
separation factor (anthracene/progesterone): 1.90 - 2.35

Informations légales

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificats d'analyse (COA)

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Articles

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

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