14-864
PAK-1 PBD Protein (agarose free), 300 µg
For use in Affinity Binding Assays, ELISA & G-Protein Assays.
Synonyme(s) :
p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1
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About This Item
Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Niveau de qualité
Poids mol.
Mw 21 kDa
Fabricant/nom de marque
Upstate®
Concentration
>50% (Coomassie blue staining, SDS-PAGE)
2 mg/mL
Technique(s)
ELISA: suitable
affinity binding assay: suitable (G-protein)
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Description générale
Product Source: GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1, expressed in E. coli.
Qualité
Routinely evaluated by G-protein activity assay
Forme physique
300 µg of PAK-1 PBD, GST in 150 µL of PBS, pH 7.4, 0.5 mM PMSF, containing 20% glycerol.
Stockage et stabilité
Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Remarque sur l'analyse
Specific Activity: Affinity Precipitation Assay: 10 µg of this lot when added with 10 µL glutathione agarose, precipitated Rac-GTP from 3T3 and A431 cell lysates that were loaded in vitro with GTPγS. GDP treated of 3T3 and A431 lysates served as negative controls. Precipitated Rac-GTP was detected by immunoblot analysis using 1 &micor;g/mL anti-Rac (Catalog # 05-389) and secondary goat anti-mouse HRP antibody (Catalog # 12-349) on a Western Blot.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
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Shamsideen A Ojelade et al.
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V Benard et al.
The Journal of biological chemistry, 274(19), 13198-13204 (1999-05-01)
A major function of Rac2 in neutrophils is the regulation of oxidant production important in bacterial killing. Rac and the related GTPase Cdc42 also regulate the dynamics of the actin cytoskeleton, necessary for leukocyte chemotaxis and phagocytosis of microorganisms. Although
Yuna Guo et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 21(22), 5064-5072 (2015-06-14)
We previously identified the R-enantiomer of ketorolac as an inhibitor of the Rho-family GTPases Rac1 and Cdc42. Rac1 and Cdc42 regulate cancer-relevant functions, including cytoskeleton remodeling necessary for tumor cell adhesion and migration. This study investigated whether administration of racemic
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