14-429-M

MEK1 Protein, active, 10 µg

Active, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

• Synonyme(s) : CFC3, MAP2K1, MAPKK1, MEL, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1
• Code UNSPSC : 12352200
• eCl@ss : 32160405
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Gamme de produits: Upstate^®
• Forme: liquid
• Activité spécifique: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• Durée de conservation: 1 yr
• Poids mol.: M_w 71 kDa
• Produit purifié par: (Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose)
• Espèces réactives: human
• Fabricant/nom de marque: Upstate^®
• Technique(s): activity assay: suitable (kinase)
• Solubilité: soluble
• Numéro d'accès: (FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. ); NM_002755.2
• Numéro d'accès NCBI: NM_002755.2
• Numéro d'accès UniProt: Q02750
• Température de stockage: −70°C
• Informations sur le gène: human ... MAP2K1(5604)

Description

Description générale

Research area: Cell Signaling Protein Target: MEK1 Target Sub-Family: STE

N-terminal GST and Cterminal His6-tagged, recombinant full-length human MEK1

Actions biochimiques/physiologiques

Mitogen-activated protein kinase kinase 1 (MEK1) is a hydrophilic non-receptor protein kinase that shows ubiquitous expression across different cell types. It serves as a critical component of the RAS-RAF-MEK-ERK signal transduction cascade, also known as the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) cascade. This signaling pathway plays a crucial role in regulating various biological processes, including apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Additionally, MEK1 protein contributes to the development of the extra-embryonic ectoderm during placentogenesis. Somatic mutations in the MEK1 gene have been observed in individuals with melanoma, a form of skin cancer.

Conditionnement

Also available in 10 μg size and in bulk

Also available in 250μg size as catalog # 14-429M - Call for pricing and availability.

Qualité

routinely evaluated by phosphorylation of MBP

Description de la cible

MEK1

Forme physique

Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose

Stockage et stabilité

1 year at -70°C

Autres remarques

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317
• Conseils de prudence: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classification des risques: Skin Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°C): Not applicable