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Merck
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Principaux documents

126593

Sigma-Aldrich

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade

Synonyme(s) :

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade

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About This Item

Numéro CAS:
9048-46-8
Numéro MDL:
MFCD00011344
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.25

Description

Merck USA index - 14, 8468

Essai

≥98% (agarose gel electrophoresis)

Forme

solid

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Impuretés

≤3.0 ash
≤5.0% moisture

Couleur

off-white

Solubilité

0.15 M sodium chloride: 10 mg/mL

Traces de cations

heavy metals: ≤10 ppm

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade is a purified plasma protein that is useful as a stabilizing agent and as a carrier protein. It is prepared under non-denaturing conditions by modification of the Cohn procedure and caprylic acid heat-shock treatment.
Bovine serum albumin (BSA) is an α-helical, non-glycosylated globular protein with 17-disulfide bonds.

Application

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade has been used:
  • to block tissue sections of placenta in immunohistochemical (IHC) analysis
  • as a component of Krebs buffer for in vitro glucose-stimulated insulin secretion (GSIS) experiments and drug/palmitate preparation
  • as a vehicle control to incubate whole mouse blood to study plate aggregation in vivo

Actions biochimiques/physiologiques

Bovine Serum Albumin (BSA), an important component in the cell culture media can transport fatty acids, hormones, and drugs. It is generally used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Olga Troitskaya et al.
International journal of molecular sciences, 22(23) (2021-12-11)
Multicellular spheroids with 3D cell-cell interactions are a useful model to simulate the growth conditions of cancer. There is evidence that in tumor spheroids, the expression of various essential molecules is changed compared to the adherent form of cell cultures.
Amber Lockridge et al.
Cell reports, 31(5), 107609-107609 (2020-05-07)
During early obesity, pancreatic β cells compensate for increased metabolic demand through a transient phase of insulin hypersecretion that stabilizes blood glucose and forestalls diabetic progression. We find evidence that β cell O-GlcNAcylation, a nutrient-responsive post-translational protein modification regulated by
Tamara Topală et al.
Clujul medical (1957), 87(4), 215-219 (2014-01-01)
The continuous search for new molecules with therapeutic abilities has led to the synthesis and characterization of a large number of metal complexes, proven to exhibit potential as pharmacological agents through their antibacterial, antiviral, antifungal and antineoplastic properties. As serum
Yuhong Xiao et al.
Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter

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