07-217-I

Anti-NAG-1/GDF15

from rabbit, purified by affinity chromatography

• Synonyme(s) : Macrophage inhibitory cytokine 1, MIC-1, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1, Placental TGF-beta, Placental bone morphogenetic protein, Prostate differentiation factor
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_004855
• Numéro d'accès UniProt: Q99988
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Growth/differentiation factor 15 (UniProt: Q99988; also known as GDF-15, Macrophage inhibitory cytokine 1, MIC-1, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1, Placental TGF-beta, Placental bone morphogenetic protein, and Prostate differentiation factor) is encoded by the GDF15 (also known as MIC1, PDF, PLAB, PTGFB) gene (Gene ID: 9518) in human. NAG-1 is a member of the TGF-beta super family, however, it does not display strong homology to existing families. NAG-1 is synthesized as a 308-amino acid pro-NAG-1 monomer and then dimerizes by a specific disulfide linkage. The pro-NAG-1 dimer is then cleaved by furin-like proteases at an RXXR site, forming a 112 amino acid C-terminal dimeric protein and pro-peptide. It is expressed in placenta and is found in lower levels in prostate, kidney, and colon. Its expression is significantly increased during inflammation, injury, and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. NAG-1 expression is also shown to increase following exposure to a number of non-steroidal anti-inflammatory drugs. Induced expression of NAG-1 is shown to result in stimulation of apoptosis and inhibition of cell growth.

Spécificité

This polyclonal antibody specifically detects Growth/differentiation factor 15 in human.

Immunogène

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal end of human NAG-1/GDF15.

Application

Detect Growth/differentiation factor 15 using this rabbit polyclonal Anti-NAG-1/GDF15, Cat. No. 07-217-I, useing Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 2 µg/mL from a representative lot detected NAG-1/GDF15 in 10 µg of HepG2 conditioned media.



Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NAG-1/GDF15 in human placenta tissue.

Qualité

Evaluated by Western Blotting in LNCap Conditioned Media.

Western Blotting Analysis: 2 µg/mL of this antibody detected NAG-1/GDF15 in 10 µg of LNCap Conditioned Media.

Description de la cible

34.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Liaison

Replaces: 07-217

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1