07-2130

Anti-Ubiquitin Antibody

from rabbit

• Synonyme(s) : Polyubiquitin-C, Ubiquitin
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: rat, human, mouse
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NP_066289
• Numéro d'accès UniProt: P0CG48
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... UBC(7316)

Description

Description générale

Ubiquitin functions as a marker to indicate the fate of other proteins in the cell. It can be attached as a single molecule or as a polyubiquitin chain. Although ubiquitinated proteins are oftentimes tagged for degradation by the 20S proteosome, ubiquitination can also indicate alternative fates depending on the lysine residue that is involved in linking the ubiquitin chain to the target protein.Ubiquitin chains linked by Lys33 to kinase substrates such as AMP-activated protein kinases, (AMPKα1, BRSK1, MARK3, MARK4, and NUAK1), may activate or deactivate these proteins, supporting the role for uniquitin in regulating signaling.

Spécificité

Broad species reactivity expected based on sequence similarity.

This antibody recognizes Ubiquitin at the N-terminus

Immunogène

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human Ubiquitin.

Application

Research Category
Signaling

Research Sub Category
General Post-translation Modification

This Anti-Ubiquitin antibody is validated for use in IP & WB for the detection of the Ubiquitin protein.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Ubiquitin on 10 µg of HeLa cell lysate.

Qualité

Evaluated by Immunoprecipitation in serum starved HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody detected Ubiquitin on 5 µL of serum starved HeLa cell lysate.

Description de la cible

~8 kDa observed. The calculated molecular weight of this protein is 8 kDa, but has been observed at ~24 kDa due to tetramers. (Kvint, K., et al. (2008). Mol Cell.30(4):498-506.)

Forme physique

Affinity purified

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Serum starved HeLa cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable