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07-1735

Sigma-Aldrich

Anti-HuR Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

ELAV-like 1, Hu antigen R, Hu-antigen R, embryonic lethal, abnormal vision, drosophila, homolog-like 1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human, rat, mouse

Technique(s)

immunofluorescence: suitable
western blot: suitable

Numéro d'accès NCBI

NP_001410.2

Numéro d'accès UniProt

Q15717

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ELAVL1(1994)
mouse ... Elavl1(15568)
rat ... Elavl1(363854)

Description générale

HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK.

Spécificité

Detects HuR
Other species have not been tested.

Immunogène

Epitope: N-terminus
Synthetic peptide corresponding to the N-terminus of human HuR.

Application

Immunofluorescent ICC Analysis:
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

RNA Binding Protein (RBP)
Use Anti-HuR Antibody (Rabbit Polyclonal Antibody) validated in WB, IF to detect HuR also known as ELAV-like 1, Hu antigen R.

Qualité

Western Blot Analysis:
The antibody detected HuR at 1:500-1:1,000 dilutions in Jurkat cell lysate resolved via SDS-PAGE and transferred to PVDF.

Description de la cible

36 kDa

Liaison

Replaces: AB5831

Forme physique

Affinity purified polyclonal precipitated in ammonium sulfate.
Rabbit polyclonal precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Remarque sur l'analyse

Control
Jurkat cell lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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