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Key Documents

07-1562

Sigma-Aldrich

Anti-EZH2 Antibody

serum, Upstate®

Synonyme(s) :

SETDB1, EC 2.1.1.43

SET domain bifurcated 1

ERG-associated protein with SET domain

ESET

Histone H3-K9 methyltransferase 4

H3-K9-HMTase 4

Lysine N-methyltransferase 1E

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

rat, mouse, human

Fabricant/nom de marque

Upstate®

Technique(s)

western blot: suitable

Numéro d'accès GenBank

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... EZH2(2146)

Description générale

Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro.. EZH2, also known as ENX-1, is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis and placenta. EZH2 and BMI-1 genes are co expressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene.

Spécificité

Recognizes EZH2, Mr ~100 kDa

Immunogène

KLH-conjugated, His-tagged fusion protein corresponding to residues 406-746 of human EHZ2 (similar to enhancer of zeste (Drosophila) homolog 2). Based on accession # BC010858.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Histones
Use Anti-EZH2 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EZH2 also known as SET domain bifurcated 1, ERG-associated protein with SET domain.

Qualité

Routinely evaluated by immunoblot analysis

Description de la cible

95-100kDa

Liaison

Replaces: 04-1047

Forme physique

Serum

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
A431 cell lysate

Informations légales

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2


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