07-1404

Anti-GLUT-4 Antibody, C-terminus

from rabbit, purified by affinity chromatography

• Synonyme(s) : Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, GLUT-4
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse, human
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology)
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_001033
• Numéro d'accès UniProt: P14672
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SLC2A4(6517)

Description

Description générale

The protein called GLUT-4, or more correctly Solute carrier family 2, facilitated glucose transporter member 4 and encoded by the human gene SLC2A4/GLUT4 is a primary insulin regulated facilitative glucose transporter found primarily in skeletal and cardiac muscle, as well as brown and white fat but is also expressed in other tissues such as brain. In the brain GLUT4 is found exclusively in neurons. GLUT-4 is facilitative because it is not always present on the cell plasma membrane. GLUT-4 only localizes to the plasma membrane upon insulin induction. GLUT-4 is localized to the perinuclear region in resting cells. Mutations in GLUT-4 are a cause of one form of non-insulin dependent diabetes mellitus. GLUT4 function as well as its transcriptional regulation is highly conserved throughout the vertebrate species.

Spécificité

Glucose transporter 4 (GLUT-4) is a protein present in rat skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species, and the peptide sequence selected for production of this antibody is identical in rat, human (amino acids 498-510), and mouse. This antibody shows no cross-reactivity of HepG2 type transporter from rat brain or other glucose transporters.

Immunogène

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human GLUT-4.

Application

Detect GLUT-4 using this Anti-GLUT-4 Antibody, C-terminus validated for use in WB, IH(P), ELISA.

Immunohistochemistry Analysis: A 1:50-2,000 dilution from a representative lot detected GLUT-4 in human cardiac myocytes and human skeletal muscle myocytes.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Qualité

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLUT-4 in 10 µg of NIH3T3 cell lysate.

Description de la cible

~58 kDa observed

Liaison

Replaces: AB1346

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
NIH3T3 cell lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2