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Key Documents

06-659

Sigma-Aldrich

Anti-phospho-c-Jun (Ser73) Antibody

Upstate®, from rabbit

Synonyme(s) :

Activator protein 1, Jun activation domain binding protein, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, enhancer-binding protein AP1, jun oncogene, v-jun sarcoma virus 17 oncogene homolog, v-jun sarcoma virus 17 oncogene homolog

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

mouse, human

Réactivité de l'espèce (prédite par homologie)

Xenopus (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), zebrafish (based on 100% sequence homology)

Fabricant/nom de marque

Upstate®

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Numéro d'accès NCBI

NP_002219.1

Numéro d'accès UniProt

P05412

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

phosphorylation (pSer73)

Informations sur le gène

human ... JUN(3725)
mouse ... Jun(16476)
rat ... Jun(24516)
zebrafish ... Jun(335916)

Description générale

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced

signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

Spécificité

This antibody detects phospho-c-Jun. May also detect phospho-JunD in properly treated cells since the sequence homology is identical (13/13).

Immunogène

KLH-conjugated linear peptide corresponding to the human Phospho-C-Jun (Ser73) near the N-terminus.

Application

Anti-phospho-c-Jun (Ser73) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-c-Jun (Ser73) also known as Activator protein 1 & has been validated in ICC, IP & WB.
Western Blot Analysis :
A previous lot of this antibody detected 10 ng of phosphorylated gst-Jun, but did not detect 10 ng of nonphosphorylated Jun, as reported by an independent laboratory.

Qualité

Evaluated by Western Blotting in Anisomycin treated NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Phospho-C-Jun (Ser73) in 10 µg of Anisomycin treated NIH/3T3 cell lysate.

Description de la cible

~40 kDa

Liaison

Replaces: 04-213

Forme physique

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.

Remarque sur l'analyse

Control
Untreated and anisomycin-treated NIH/3T3 cell lysate.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

C Urbich et al.
The Journal of clinical investigation, 108(10), 1451-1458 (2001-11-21)
Atherosclerosis is an inflammatory disease of large arteries that is initiated through the activation of endothelium by proinflammatory mediators. CD40 receptor stimulation has been implicated in the pathogenesis of atherosclerosis. One of the most important atheroprotective stimuli is the viscous
Carlos O Stocco et al.
The Journal of biological chemistry, 277(5), 3293-3302 (2001-11-24)
We have previously demonstrated that prostaglandin F(2alpha) (PGF(2alpha)) induces a rapid and transient expression of Nur77 in luteal cells. We have shown that Nur77 plays an important role in ovarian physiology by mediating the PGF(2alpha) induction of 20alpha-HSD, a steroidogenic
Raf-independent deregulation of p38 and JNK mitogen-activated protein kinases are critical for Ras transformation.
Pruitt, K; Pruitt, WM; Bilter, GK; Westwick, JK; Der, CJ
The Journal of Biological Chemistry null
M Repici et al.
Neuroscience, 159(1), 94-103 (2009-01-13)
The c-Jun N-terminal kinase (JNK) is a mitogen-activated protein kinase (MAPK) activated by stress-signals and involved in many different diseases. Previous results proved the powerful effect of the cell permeable peptide inhibitor d-JNKI1 (d-retro-inverso form of c-Jun N-terminal kinase-inhibitor) against
Stable expression of intracellular Notch suppresses v-Src-induced transformation in avian neural cells.
Mateos, S; Amarir, S; Laugier, D; Marx, M; Calothy, G
Oncogene null
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