06-1002

Anti-LAP2 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : LEM domain containing 4, TP alpha, TP beta/gamma, TPRP isoform alpha, TPRP isoforms beta/gamma, Thymopoietin isoform alpha, Thymopoietin, isoforms beta/gamma, Thymopoietin-related peptide isoform alpha, Thymopoietin-related peptide isoforms beta/gamma, l
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: rat, human, mouse, canine
• Technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Numéro d'accès NCBI: NM_003276
• Numéro d'accès UniProt: P42167
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TMPO(7112)

Description

Description générale

The inner membrane of the nuclear envelope is connected to the nuclear lamina. The main components of the nuclear lamina are Type V intermediate filaments called lamins and lamin binding proteins including lamin B receptor, emerin, MANI, LAP1 (3 isoforms), and LAP2 (several isoforms). The lamins are divided into two groups: the constitutively expressed lamin B and the developmentaly regulated lamin A.
The best characterized isoforms of lamin binding protein LAP2 are LAP2α and LAP2β. LAP2β is a type II membrane protein in the inner nuclear membrane that binds lamin B and is important to cell viability and controlling nuclear lamina growth. LAP2α has been characterized as a nucleoplasmic protein that interacts with A-type lamins to control gene expression, transcription, and chromatin organization.

Spécificité

Predicted to cross-react with canine (dog) and rat based on sequence homology.

This antibody recognizes LAP2.

Immunogène

KLH-conjugated synthetic linear peptide corresponding to LAP2.

Application

Anti-LAP2 Antibody is an antibody against LAP2 for use in WB, IC & IF.

Immunofluorescence:
Representative lot data. This antibody was used to detect the nuclear lamina by immunofluorescence.

Immunocytochemistry:
Representative lot data.
A previous lot was used in confocal fluorescent analysis of A431, HeLa and NIH/3T3 cells using anti-LAP2 rabbit polyclonal antibody (Red).
Actin filaments have been labeled with AlexaFluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cytoskeletal Signaling

Qualité

Evaluated by western blot on HeLa cell lysate.

Western Blot Analysis:
A 1:1000-1:3000 dilution of this antibody was used to detect LAP2 in HeLa cell lysate.

Description de la cible

~55 kDa

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8ºC from date of receipt.

Remarque sur l'analyse

Control
HeLa cell lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable