05-988

Anti-Pras40 Antibody, Clone 73P21

clone 73P21, Upstate^®, from mouse

• Synonyme(s) : 40 kDa proline-rich AKT substrate, AKT1 substrate 1 (proline-rich), proline-rich Akt substrate, 40 kDa
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 73P21, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Upstate^®
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NP_001092102
• Numéro d'accès UniProt: Q96B36
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... AKT1S1(84335)

Description

Description générale

PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.

Spécificité

Recognizes PRAS40.

This antibody does not cross react with mouse.

Immunogène

Recombinant human PRAS40 (full-length) expressed in E. coli.

Application

Anti-Pras40 Antibody, Clone 73P21 is an antibody against Pras40 for use in IP & WB.

Immunoblot Analysis: A 01-1.0 μg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
PI3K, Akt, & mTOR Signaling

Qualité

Routinely evaluated by western blot.

Western Blot Analysis:
A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.

Description de la cible

40 kDa

Forme physique

Affinity purified

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.1% sodium azide.

Stockage et stabilité

Stable for 1 years at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
RIPA lysates of HeLa cells.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable