05-623-AF488

Anti-RNA polymerase II Antibody, clone CTD4H8, Alexa Fluor^™ 488 Conjugate

clone CTD4H8, from mouse, ALEXA FLUOR^™ 488

• Synonyme(s) : DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 488
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: CTD4H8, monoclonal
• Espèces réactives: human, mouse
• Réactivité de l'espèce (prédite par homologie): yeast (based on 100% sequence homology), rat (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NP_000928.1
• Numéro d'accès UniProt: P24928
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... POLR2A(5430) ; mouse ... Polr2A(20020) ; rat ... Polr2A(363633)

Description

Description générale

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in HeLa cells.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.

This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor 488 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.

Qualité

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in A431 cells.

Description de la cible

~210-220 kDa observed

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable