G004631
HPLC Analysis of Methylphenobarbital (Mephobarbital) Enantiomers on Astec® CHIROBIOTIC® V
application for HPLC
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About This Item
technique(s)
HPLC: suitable
test parameters
column: CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase: [A] ethanol; [B] hexane; (25:75, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 205 nm
injection: 10 μL
sample: 1 mg/mL in methanol
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
Analysis Note
Methylphenobarbital (also known as mephobarbital and mephobarbitone) is a barbiturate derivative used primarily as an anticonvulsant, but also as a sedative and anxiolytic. It is a chiral compound and the enantiomers have been shown to exhibit different bioactivity, one narcotic, one convulsant, neither of which is seen in the racemate. Shown here is the rapid, efficient separation of methylphenobarbital enantiomers on an Astec CHIROBIOTIC V column. Highest grade HPLC solvents were used to supply low background interference and low particulate contamination for robust, trouble-free operation. A Sigma-Aldrich USP reference standard provided reliable identification and quantification.
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
Analyte
Description
Mephobarbital, United States Pharmacopeia (USP) Reference Standard
analytical column
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