G003784

GC Analysis of Volatiles in Roasted Coffee Beans on Omegawax after SPME using a 50/30 μm DVB/Carboxen/PDMS Fiber

application for SPME, suitable for GC

Properties

• technique(s): gas chromatography (GC): suitable; solid phase microextraction (SPME): suitable
• test parameters: sample/matrix: 2 g of coffee beans; SPME fiber: 50/30 μm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber (57329-U); extraction: headspace, with a pre-equilibration period of 10 min. at 60 °C, and then a fiber exposure of 40 min. at 60 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 rpm; desorption process: 260 °C for 5 min; column: Omegawax 250, 30 m x 0.25 mm I.D., 0.25 μm (24136); oven: 40 °C (5 min), 4 °C/min to 230 °C, 50 °C/min to 280 °C (2 min); inj. temp.: 260 °C; detector: MS, m/z 40-400; carrier gas: helium, 34.8 cm/sec constant; injection: splitless; scan range: m/z 40-400; sample: Roasted coffee; liner: 0.75 mm I.D. SPME/Splitless (2633901)
• suitability: application for SPME
• application(s): food and beverages

Description

Analysis Note

Headspace-SPME followed by GC analysis was used in this application to analyze volatile compounds in roasted coffee. SPME is ideal for analyzing the headspace of solid samples like coffee beans. The Carboxen/DVB/PDMS fiber is the fiber of choice for analyzing the wide range of analytes commonly found in roast coffee beans.The relatively polar Omegawax 250 GC column provided high resolution.

Legal Information

Analyte

Methyl acetate
2-Methylfuran
Ethyl acetate
2-Butanone
2,3-Dihydro-5-methylfuran
Ethyl alcohol
2,5-Dimethylfuran
2,3-Butanedione
3-Hexanone
2,3-Pentanedione
Hexanal
3-Penten-2-one
2,3-Hexanedione
1-Methylpyrrole
3,4-Hexanedione
α-Terpinene
Pyridine
(R)-(+)-Limonene, purum, ≥98.0% (sum of enantiomers, GC)
Pyrazine
γ-Terpinene
2-Methylpyrazine
p-Cymene
2,5-Dimethylpyrrole
Acetoin, primarily dimer, ≥95%, FG
2,5-Dimethylpyrazine
2,6-Dimethylpyrazine
2-Ethylpyrazine, ≥98%, FG
2,3-Dimethylpyrazine
4-Heptanone
3-Ethylpyridine
2,3,5-Trimethylpyrazine
Furfural
Pyrrole
Furfuryl acetate
Linalool
Linalyl acetate
5-Methylfurfural
γ-Butyrolactone
2-Acetyl-1-methylpyrrole
Furfuryl alcohol
2-Acetylpyrrole
Pyrrole-2-carboxaldehyde
See All (42)