G003781

GC Analysis of Volatiles in Limoncello on SLB^®-5ms after SPME using a 100 μm PDMS Fiber

application for SPME, suitable for GC

Properties

• technique(s): gas chromatography (GC): suitable; solid phase microextraction (SPME): suitable
• test parameters: sample/matrix: Limoncello diluted 1:100 in water, then 5 mL of this solution transferred to a 10 mL crimped vial spiked with 100 μL of a 100 ppm solution of nonane (internal standard); SPME fiber: 100 μm polydimethylsiloxane (57300-U); extraction: headspace, with a pre-equilibration period of 10 min. at 40 °C, and then a fiber exposure of 30 min. at 40 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 ppm; desorption process: 250 °C for 5 min; column: SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U); oven: 40 °C, 3 °C/min to 250 °C (2 min); inj. temp.: 250 °C; detector: FID, 260 °C (sampling rate 80 ms); carrier gas: helium, 35.0 cm/sec; injection: splitless; liner: 0.75 mm I.D. SPME (2633901); sample: Limoncello liquor
• suitability: application for SPME
• application(s): food and beverages

Description

Analysis Note

In this application, SPME was used for the analysis of volatiles in limoncello using a headspace method. SPME is ideal for the analysis of aroma volatiles in flavored beverages such as liquors. A 100 μm PDMS fiber was inserted into the headspace of the limoncello sample for 30 minutes at 40 °C followed by GC analysis using FID detection. The SLB-5ms GC columns provide consistently low-bleed, inert, efficient, and durable separations.

Legal Information

SLB is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Tricyclene
α-Pinene
Camphene
Myrcene
Octanal
α-Terpinene
p-Cymene
(R)-(+)-Limonene, purum, ≥98.0% (sum of enantiomers, GC)
γ-Terpinene
Terpinolene
Linalool
Nonanal
(−)−α-Terpineol, natural, ≥96%, FCC, FG
Decanal
Octyl acetate
Neral
Geraniol
Geranial
Isobornyl acetate
Undecanal
Nonyl acetate
Citronellyl acetate
Neryl acetate
3,7-Dimethyl-2,6-octadienyl acetate
α-Humulene
Geranyl propionate
α-Phellandrene
Valencene, natural, ≥65%
α-Bisabolol
See All (29)