58338
SUPELCOSIL™ LC-NH2 HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mm
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About This Item
Recommended Products
Agency
suitable for USP L8
Quality Level
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
extent of labeling
3% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 5.1 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
amino phase
particle size
5 μm
pore size
120 Å
application(s)
food and beverages
separation technique
hydrophilic interaction (HILIC)
normal phase
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General description
Silica-based SUPELCOSIL LC-NH2 columns separate monosaccharides, disaccharides, and some trisaccharides, with elution generally in order of increasing molecular weight. Retention decreases as the proportion of water:acetonitrile in the mobile phase is increased. For more information, request T397126 (Sugars on SUPELCOSIL LC-NH2 Columns).
The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petroleum industry (see SUPELCOSIL™ LC-NH2-NP HPLC Columns for additional details).
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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Protocols
A protocol discussing HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2
Chromatograms
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