ZRB2146

Anti-Cyclophilin B Antibody, clone 4D5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Peptidyl-prolyl cis-trans isom+C4erase B;EC:5.2.1.8;PPIase B;CYP-S1;Rotamase B;S-cyclophilin; SCYLP
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4D5, recombinant monoclonal
• description: 4D5 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 23.74 kDa; observed mol wt ~18 kDa
• purified by: using Protein A
• species reactivity: human, monkey, rat, mouse
• species reactivity (predicted by homology): porcine, bovine, feline, canine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_000933
• UniProt accession no.: P23284
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CYPB(5479)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 4D5 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Cyclophilin B. It targets an epitope within 17 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Cyclophilin B.

Application

Quality Control Testing

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cyclophilin B in NIH3T3 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cyclophilin B in lysates from COS-7, C6, A431, and U87 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cyclophilin B in human thyroid tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant Human Cyclophilin B with a KD of 3.6 x 10-6 in an affinity binding assay.

Enzyme Immunoassay Analysis (ELISA): A representative lot of this antibody detected recombinant Human Cyclophilin B in ELISA application.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Peptidyl-prolyl cis-trans isomerase B (UniProt: P23284; also known as EC:5.2.1.8, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP) is encoded by the CYPB (also known as PPIB) gene (Gene ID: 5479) in human. Cyclophilins are a group of peptidyl-prolyl cis/trans isomerases that play crucial role in regulatory mechanisms of cellular physiology and pathology in several inflammatory conditions. Cyclophilin B (CypB) is an endoplasmic reticulum (ER) resident protein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to produce mature active protein (aa 34-216). It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and therefore assists in protein folding. CypB is secreted by several cell types, and it possesses proinflammatory properties. It is reported to play a key role in ER redox homeostasis. Higher levels of CypB are observed in inflammatory processes, like sepsis and rheumatoid arthritis. It also promotes platelet adhesion to collagen and the neutrophil chemotaxis. It binds to the CD147 membrane receptor to modulate inflammatory pathways. CypB is reported to provide essential survival signals in glioblastoma multiforme cells. Suppression of CypB is shown to reduce the proliferation of glioblastoma cells. Depletion or pharmacological inhibition of CypB causes hyperactivation of the oncogenic RAS-mitogen-activated protein kinase pathway, induction of cellular senescence signals, and death resulting from loss of MYC, mutant p53, Chk1, and JAK/STAT3 signaling. Mutations in CYPB gene are known to cause Osteogenesis imperfecta, a disease characterized by low bone mass, bone fragility and increased susceptibility to fractures even after minimal trauma. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gegunde, S., et al. (2021). Front. Immunol. 12; 609196; Choi, JW., et al. (2014). Cancer Res. 74(2); 484-496).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable