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TRUE8

Sigma-Aldrich

TrueGel3D Hydrogel Kits

SLO-PVA, CD cell-degradable crosslinker

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

shipped in

dry ice

storage temp.

−70°C

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General description

The gel is formed by stable thioether bonds between thiol groups on CD cell-degradable crosslinker and thio-reactive groups on SLO-PVA. The polymer, SLO-PVA is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-PVA. SLO-PVA does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.
The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

Application

TRUE8-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of matrix stiffness on cell physiology and behavior. These hydrogels are also employed to study cell migration of neutrophils, dendritic cells and lymphocytes. The SLO-PVA based hydrogels are preferred over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes.

Features and Benefits

  • Slow gelling (takes 1.5 minutes to 4 minutes, depends on gel stiffness)
  • Flexibility in modulating gel stiffness
  • Contains MMP-cleavable crosslinker
  • Does not allow cell recovery and post-culture analyses
  • Cell inert hydrogel (if not customized with RGD peptide)

Components

TRUE8-1KT contains SLO-PVA solution in phosphate buffer and lyophilized CD cell-degradable crosslinker with reconstitution buffer and water.

Caution

Avoid prolonged exposure of CD cell-degradable crosslinker to air and store SLO-PVA in small portions to avoid frequent freeze-thaw cycles.

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

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Articles

True Gel3D™ Hydrogel Troubleshooting Guide

TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

Protocols

TrueGel3D™ Slow Gelling Hydrogels Preparation Protocol

TrueGel3D™ is chemically defined hydrogel, enables cell growth in 3D environment and mimics native tissue environment. Slow gelation facilitates filling of microchannels or syringes. Find detail protocol of slow gelation for TrueGel3D™ kits

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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