T0831
Tissue plasminogen activator human
Synonym(s):
tPA
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
form
lyophilized powder
Quality Level
specific activity
≥300 KU/mg protein
mol wt
~70 kDa
impurities
HIV, HCV, and HBsAg, tested negative
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... PLAT(5327)
Looking for similar products? Visit Product Comparison Guide
General description
Tissue plasminogen activator (tPA) is a protease of the S1 family (trypsin family) and is found in a wide variety of mammalian tissues, especially endothelial cells. tPA is secreted as a single chain precursor, which is cleaved to a two-chain form by plasmin. Supplied in the single chain form with a molecular weight of ~70 kDa. The Tissue Plasminogen Activator (tPA) gene is mapped to human chromosome 8p11.21 and encodes a protease of 69 kDa majorly containing serine amino acid residue. It is predominantly expressed in endothelial cells.
Application
Tissue plasminogen activator has been used in a study to assess treatment risks of intracranial hemorrhage among patients with acute ischemic stroke. Tissue plasminogen activator has also been used in a study to investigate the successful treatment of early thrombosis of HeartWare left ventricular assist devices with intraventricular thrombolytics. Human tissue plasminogen activator has been used to study the effect of tyrosinase-related protein 2 (TRP-2) expression on melanin production in sheep.
Biochem/physiol Actions
tPA is a potent activator of fibrinolysis and converts plasminogen into plasmin and also initiates cell signaling. The plasminogen activation system may also be associated with degradation of amyloid fibrils. It is also known to mediate inflammatory and extracellular matrix remodeling processes and is predicted to participate in disease progression of intracranial aneurysm indicating brain vascular malformations. Upregulation of the gene is observed in aortic aneurysms.
Physical form
Lyophilized powder, from 100 mM PBS (pH 7.4), 3.5 mg/mL L-arginine, and 0.001% Tween® 80
Preparation Note
Reconstitute with dH2O to a final concentration of 1 mg/mL (e.g. 100 μg should be reconstituted in 100 μL).
Legal Information
TWEEN is a registered trademark of Croda International PLC
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Converting Tissue Type Plasminogen Activator into a Zymogen IMPORTANT ROLE OF Lys156.
Tachias K and Madison E L
The Journal of Biological Chemistry, 272(1), 28-31 (1997)
Design, synthesis, and characterization of urokinase plasminogen-activator-sensitive near-infrared reporter.
Law B, et al.
Chemistry & Biology, 11(1), 99-106 (2004)
Successful treatment of early thrombosis of HeartWare left ventricular assist device with intraventricular thrombolytics.
Kamouh A, et al.
The Annals of Thoracic Surgery, 94(1), 281-283 (2012)
Differential expression assay of chromosome arm 8p genes identifies Frizzled-related (FRP1/FRZB) and Fibroblast Growth Factor Receptor 1 (FGFR1) as candidate breast cancer genes.
Ugolini F, et al.
Oncogene, 18(10), 1903-1903 (1999)
Amorphous protein aggregates stimulate plasminogen activation, leading to release of cytotoxic fragments that are clients for extracellular chaperones.
Constantinescu P, et al.
The Journal of Biological Chemistry (2017)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service