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SAB4600467

Sigma-Aldrich

Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, CF405M antibody produced in donkey

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

donkey

Quality Level

conjugate

CF 405M conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

guinea pig

concentration

~2 mg/mL

technique(s)

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1-10 μg/mL
western blot: suitable

fluorescence

λex 408 nm; λem 452 nm

storage temp.

−20°C

target post-translational modification

unmodified

General description

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Guinea Pig is a highly preferred laboratory animal to study the local and anaphylactic reactions. It has two subclasses of IgG- IgG1 and IgG2. IgG1 helps in complement fixation through alternate pathway and IgG2 fixes complement by conventional mechanism.
This product is prepared by labeling high quality donkey anti-guinea pig IgG (H+L) with CF405M. CF405M is spectrally similar to and as bright as Pacific Blue dye but is significantly more photostable than the latter. An additional advantage of CF405M is that it has less spillover in the 525/50 green channel than Pacific Blue dye due to narrower emission peak, making CF405M a superior choice in multi-color detection applications. To minimize cross-reactivity, the antibody has been adsorbed against Bovine, Chicken, Goat, Syrian Hamster, Horse, Human, Mouse, Rabbit, Sheep and Rat serum. 

Specificity

Binds all guinea pig IgGs (minimal cross-reaction with bovine, chicken, goat, syrian hamster, horse, human, mouse, rabbit, rat and sheep proteins)

Immunogen

guinea pig IgG (H+L)

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Preparation Note

Protect from light. The antibody solution should be gently mixed before use.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Molecular Genetics of Immunoglobulin, 17 (1987)
The Immunoglobulins: Structure and Function (1998)
Immunopharmacology: Proceedings of the Third International Pharmacological Meeting, 11 (2017)
Specific IgG for cat allergens in patients with allergic conjunctivitis.
Miyama A, et al.
International Ophthalmology, 35(4), 575-586 (2015)

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