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SAB4504429

Sigma-Aldrich

Anti-phospho-Histone H3.1 (pSer10) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Anti-H3.1, Anti-H3/c, Anti-H3C1, Anti-H3C10, Anti-H3C11, Anti-H3C12, Anti-H3C2, Anti-H3C4, Anti-H3C6, Anti-H3C8, Anti-H3FC, Anti-HIST1H3C

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 15 kDa

species reactivity

human, rat, mouse

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

P68431
P84243
Q71DI3

UniProt accession no.

P68431

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer10)

Gene Information

human ... HIST1H3A(8350) , HIST1H3B(8358) , HIST1H3C(8352) , HIST1H3D(8351) , HIST1H3E(8353) , HIST1H3G(8355) , HIST1H3H(8357) , HIST1H3I(8354) , HIST1H3J(8356) , HIST1H4A(8359)

General description

Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A). H3.1 is also called as HIST1H3E. It is mainly expressed in the S phase. HIST1H3E is located on human chromosome 6p22.

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Ser10.

Immunogen Range: 1-50

Biochem/physiol Actions

Histone proteins are basic building blocks of chromatin. Mutations in histone H3 results in adult cerebellar high-grade gliomas. It controls protein-protein interactions to induce binding of trans-acting factors that drive chromatin condensation. H3.1 acts as a replication-dependent histone.

Features and Benefits

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Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Comprehensive View of the Epigenetic Landscape Part I: DNA Methylation, Passive and Active DNA Demethylation Pathways and Histone Variants.
Sadakierska-Chudy A, et al.
Neurotoxicity Research, 27(1), 84?97-84?97 (2015)
Mario A Miranda et al.
Physiological reports, 8(20), e14573-e14573 (2020-10-29)
Maintenance of functional β-cell mass is critical to preventing diabetes, but the physiological mechanisms that cause β-cell populations to thrive or fail in the context of obesity are unknown. High fat-fed SM/J mice spontaneously transition from hyperglycemic-obese to normoglycemic-obese with
Selective methylation of histone H3 variant H3. 1 regulates heterochromatin replication.
Jacob Y, et al.
Science, 343(6176), 1249-1253 (2014)
Signaling to chromatin through histone modifications.
Cheung P, et al.
Cell, 103(2), 263-271 (2000)
Histone H3 K27M mutations in adult cerebellar high-grade gliomas.
Nakata S, et al.
Brain Tumor Pathology, 34(3), 113-119 (2017)
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