SAB4200683
Anti-Human IgG4 antibody, Mouse monoclonal
clone HP-6025, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Human IgG4 antibody produced in mouse
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HP-6025, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~1 mg/mL
technique(s)
immunoblotting: suitable
indirect ELISA: 0.15-0.3 μg/mL using 1 μg/ml human IgG4 myeloma proteins for coating.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Human IgG4 (mouse IgG1 isotype) is derived from the HP-6025 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgG4 is a subclass of IgG with γ4 heavy chain.
Specificity
Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.
Immunogen
Purified human IgG4 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads
Application
The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.
Biochem/physiol Actions
IgG2 and IgG4 deficiency is associated with IgA deficiency as found in patients of ataxia telangiectasia. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and thus may assist in the diagnosis of various disorders. IgG subclass expression pattern in malaria patients reveals positive association of IgG4 levels and malaria antigens but not with patients age.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Van der Meulen, FW., et al.,
British Journal of Haematology, 46, 47-56 (1980)
Aucouturier P., et al.,
Clinical and Experimental Immunology, 68, 392-36 (1987)
A Nasr et al.
Scandinavian journal of immunology, 74(4), 390-396 (2011-06-08)
The anti-malarial IgG immune response during the lengthy and dry season in areas of low malaria transmission as in Eastern Sudan is largely unknown. In this study, ELISA was used for the measurement of pre-existing total IgG and IgG subclasses
J D Isaacs et al.
Clinical and experimental immunology, 106(3), 427-433 (1996-12-01)
It is traditionally held that human IgG4 MoAbs should not deplete target cells in vivo, as this isotype is inactive in a number of in vitro assays that measure effector function. We have previously challenged this dogma, and the current
A M Walker et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(8), 3278-3282 (1995-04-11)
Several lines of evidence indicate that immunoglobulin-bound prolactin found in human serum is not a conventional complex between an anti-prolactin antibody and prolactin but a different type of association of prolactin with the Fab portion of IgG heavy chains. The
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