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Key Documents

P9791

Sigma-Aldrich

Potassium phosphate monobasic

for molecular biology, ≥98.0%

Synonym(s):

Monopotassium phosphate, Potassium dihydrogen phosphate, prim.-Potassium phosphate

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About This Item

Linear Formula:
KH2PO4
CAS Number:
Molecular Weight:
136.09
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.21

grade

for molecular biology

Quality Level

Assay

≥98.0%

form

powder

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

mp

252.6 °C (lit.)

solubility

H2O: 100 mg/mL

density

2.338 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤10 ppm

cation traces

Fe: ≤20 ppm
heavy metals (as Pb): ≤10 ppm

application(s)

diagnostic assay manufacturing

foreign activity

DNase, Exonuclease; NICKase, Endonuclease; RNase and protease, none detected

SMILES string

[K+].OP(O)([O-])=O

InChI

1S/K.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

GNSKLFRGEWLPPA-UHFFFAOYSA-M

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Application

Potassium phosphate monobasic has been used:
  • in high-throughput fluorescence assay of cytochrome P450 3A4
  • in morphological analysis of neuromuscular junction development and degeneration in rodent lumbrical muscles
  • in the measurement of net high-affinity urea uptake in maize plants

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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High-throughput fluorescence assay of cytochrome P450 3A4
Cheng Q, et al.
Nature Protocols, 4(9), 1258-1258 (2009)
Measurement of net high-affinity urea uptake in maize plants
Zanin L, et al.
BMC plant biology (2014)
Morphological analysis of neuromuscular junction development and degeneration in rodent lumbrical muscles
Sleigh J N, et al.
Journal of Neuroscience Methods, 227, 159-165 (2014)
Przemyslaw Nogly et al.
IUCrJ, 2(Pt 2), 168-176 (2015-04-14)
Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of
Leo Syrjänen et al.
Frontiers in zoology, 12, 19-19 (2015-08-25)
Carbonic anhydrases (CAs, EC 4.2.1.1) are ubiquitous enzymes that catalyze the reversible hydration reaction of carbon dioxide. CAs are present as six structurally divergent enzyme families: α, β, γ, δ, ζ and η. β-CAs have a wide distribution across different

Protocols

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry

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