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N3262

SAFC

NCTC 135 Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.13573 g/L
glucose: 1.0 g/L
phenol red: 0.02 g/L

shipped in

ambient

storage temp.

2-8°C

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General description

NCTC 135 Medium is ideal for serum-free growth of mouse L-cells.

Application

NCTC 135 Medium has been used:
  • to culture Philophthalmus sp. rediae in vitro
  • to culture Ehrlich ascites mouse tumor cell line
  • as a culture media for the differentiation of Rcho-1 cells

Quantity

Formulated to contain 9.3 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sodium bicarbonate powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

S5761

Sodium bicarbonate

Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
Aiko Aoki et al.
The American journal of pathology, 188(11), 2474-2486 (2018-08-31)
Hypertensive disorder of pregnancy (HDP) is a serious pregnancy complication that is life threatening to both the mother and fetus. Understanding HDP pathophysiology is important for developing medical treatments. This study demonstrates the involvement of autophagy deficiency in adverse maternal

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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