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N1895

Sigma-Aldrich

4-Nitrophenyl α-D-xylopyranoside

α-xylosidase substrate, ≥99% (HPLC), powder

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About This Item

Empirical Formula (Hill Notation):
C11H13NO7
CAS Number:
10238-28-5
Molecular Weight:
271.22
EC Number:
233-572-7
MDL number:
MFCD00067649
UNSPSC Code:
12352204
PubChem Substance ID:
24897536
NACRES:
NA.32

product name

4-Nitrophenyl α-D-xylopyranoside, α-xylosidase substrate

Quality Level

100

Assay

≥99% (HPLC)

form

powder

solubility

methanol: soluble 20 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

OC1COC(Oc2ccc(cc2)N(=O)=O)C(O)C1O

InChI

1S/C11H13NO7/c13-8-5-18-11(10(15)9(8)14)19-7-3-1-6(2-4-7)12(16)17/h1-4,8-11,13-15H,5H2

InChI key

MLJYKRYCCUGBBV-UHFFFAOYSA-N

Substrates

Chromogenic substrate for α-xylosidase

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

N1627

4-Nitrophenyl β-D-glucuronide

Masaki Yanagishita et al.
Kokubyo Gakkai zasshi. The Journal of the Stomatological Society, Japan, 73(1), 20-25 (2006-04-25)
Biosynthesis of proteoglycans and glycosaminoglycans in the presence of p-nitrophenyl-xyloside was studied using a primary rat ovarian granulosa cell culture system. Addition of p-nitrophenyl-xyloside into cell culture medium caused about a 700% increase of [³⁵S]sulfate incorporation (ED50 at 0.03 mM)
T Bravman et al.
FEBS letters, 495(1-2), 115-119 (2001-04-27)
A beta-xylosidase from Bacillus stearothermophilus T-6 was cloned, overexpressed in Escherichia coli and purified to homogeneity. Based on sequence alignment, the enzyme belongs to family 39 glycoside hydrolases, which itself forms part of the wider GH-A clan. The conserved Glu160
Mária Mastihubová et al.
Carbohydrate research, 339(7), 1353-1360 (2004-04-29)
Di-O-acetates and mono-O-acetates of 4-nitrophenyl beta-D-xylopyranoside were prepared by use of lipase PS-30. Polarity of organic solvents and reaction time affected the regioselectivity of the di-O-acetylation as well as the yields of monoacetates. The kinetics of acetyl groups migration in
Peter Biely et al.
Biochimica et biophysica acta, 1770(4), 565-570 (2007-01-31)
Positional specificity of NodB-like domain of a multidomain xylanase U from Clostridium thermocellum (CtAxe) was investigated. Of three monoacetates of 4-nitrophenyl beta-d-xylopyranoside the acetylxylan esterase domain showed a clear preference for the 2-acetate. Moreover, the enzyme was significantly activated by
Siyuan Li et al.
Histochemistry and cell biology, 139(1), 59-74 (2012-08-23)
Chondroitin/dermatan sulphate (CS/DS) sulphation motifs on cell and extracellular matrix proteoglycans (PGs) within stem/progenitor cell niches are involved in modulating cell phenotype during the development of many musculoskeletal connective tissues. Here, we investigate the importance of CS/DS chains and their
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