MAK503

Glucose-6-Phosphate Assay Kit

sufficient for 100 colorimetric tests

• UNSPSC Code: 12352201

Properties

• Quality Level: 100
• detection method: colorimetric
• relevant disease(s): hematological disorder
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Glucose 6-phosphate (G6P) is glucose sugar phosphorylated on carbon 6. Most of the glucose entering cells is phosphorylated to G6P. G6P has three primary fates within the cell. It lies at the start of two major metabolic pathways: glycolysis and the pentose phosphate pathway. In addition to these metabolic pathways, glucose 6-phosphate may also be converted to glycogen or starch for storage.

Features and Benefits

• Linear detection range: 10 to 1000 µM G6P.
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for glucose 6-phosphate determination in plasma, serum, tissue, cells, and culture media.

Principle

The Glucose 6-Phosphate Assay Kit provides a simple, and automation-ready procedure for measuring G6P concentration. G6P is oxidized by glucose 6-phosphate dehydrogenase and the formed NADPH is coupled to the formazan (WST-8) chromogen. The intensity of the product color, measured at 460 nm, is proportional to the G6P concentration in the sample.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H315 - H318
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
• Hazard Classifications: Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3