M0393

Medium 199

With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): M199 Medium, TCM 199
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Medium 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: HEPES: no; Hanks’ salts (2% CO₂): yes; sodium pyruvate: no; NaHCO₃: no; L-glutamine: yes; phenol red: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

• to test and determine the pH sensitivity of the sensors in a complex environment
• as a culture medium during oocyte harvesting
• as a component of Leishmania donovani (LdBOB) promastigote media for culturing L. donovani
• to culture visceral adipose tissue explants

Quantity

Formulated to contain 10.6 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable