I1146
R3 IGF-1 human
recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
Human IGF-1, IGF-1 Protein, Recombinant IGF-1
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About This Item
UNSPSC Code:
51111800
NACRES:
NA.77
Recommended Products
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥85% (HPLC)
form
lyophilized powder
potency
≤10 ng/mL ED50
quality
endotoxin tested
mol wt
7,676 Da
packaging
pkg of 0.1 mg
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... IGF1(3479)
Application
R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.
Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.
Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.
Biochem/physiol Actions
Proliferation of chinese hamster ovary CHO cells.
Other Notes
Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.
Preparation Note
Prepared from 0.1 M acetic acid
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Insulin-like Growth Factors (IGF)
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