H1506
3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni
≥15 units/mg protein
Synonym(s):
3α-Hydroxysteroid:NAD(P)+ oxidoreductase
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About This Item
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biological source
bacterial (Pseudomonas testosteroni)
Quality Level
form
lyophilized solid
specific activity
≥15 units/mg protein
purified by
chromatography
composition
Protein, ≥25%
foreign activity
β-hydroxysteroid dehydrogenase ≤0.5%
alcohol dehydrogenase ≤0.05%
storage temp.
−20°C
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Application
3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
- in the preparation of composite electrode platform to for the determination of androsterone
- in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
- for total bile salts assay using an enzymatic method
Biochem/physiol Actions
3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.
Unit Definition
One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.
Physical form
Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)
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