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Key Documents

GE17-5068-01

Sigma-Aldrich

SOURCE™ 15RPC ST 4.6/100

pkg of 1 column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

ligand

matrix surface

material

polypropylene column

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

feature

autoclavable 20 min at 121°C

packaging

pkg of 1 column

parameter

flow rate
0.5 ml/min - 2.5 ml/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
580 psi

technique(s)

HPLC: suitable

bed size

4.6 mm × 100 mm

bed volume

1.7 mL

column I.D.

4.6 mm

matrix

polymeric
polystyrene/divinylbenzene

matrix active group

polystyrene-divinylbenzene phase

avg. part. size

15 μm

cleaning

1-14

working range

1-12

capacity

~10 mg binding capacity(~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h)

suitability

suitable for bioprocess medium

separation technique

reversed phase

General description

SOURCE™ 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.

Application

SOURCE™ 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE™ 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE™ 15RPC offers properties superior to those of other polymeric matrices.

Features and Benefits

  • Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
  • Excellent scalability, from RESOURCE™ to FineLINE columns.
  • using ÄKTA® design and other high-performance liquid chromatography systems.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

RPC Purification Options and Scale up with SOURCE™ Media

The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

Protocols

Reverse Phased Chromatography (RPC) in Practice

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service