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GE17-0980-01

Butyl Sepharose 4 Fast Flow

Cytiva 17-0980-01, pack of 200 mL

Synonym(s):

Butyl Sepharose, Fast Flow Sepharose

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

butyl

packaging

pack of 200 mL

manufacturer/tradename

Cytiva 17-0980-01

matrix

4% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14

working range

3-13

suitability

suitable for bioprocess medium

General description

Butyl Sepharose 4 Fast FLow is a well established, standard aliphatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification of larger proteins.

Application

Butyl Sepharose 4 Fast FLow is part of the Sepharose Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
Butyl Sepharose 4 Fast FLow is based on cross-linked 4% Agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

Butyl Sepharose 4 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl Sepharose 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Standard aliphatic HIC media based on Sepharose Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Optimized for the separation of larger Proteins in capture and intermediate purification steps
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.

This page discusses column packing and preparation techniques for reverse phase chromatography.

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature

Protocols

This section is about the different ways of screening for selectivity of HIC medium from Cytiva, considering both automated and manual

Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.

This page discusses various aspects of sample preparation for chromatographic purification.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service