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G5080

Sigma-Aldrich

Sephadex® G-50

Fine

Synonym(s):

Gel filtration resin, Sephadex G-50 Fine Medium

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About This Item

CAS Number:
9048-71-9
UNSPSC Code:
23151817
NACRES:
NA.56

technique(s)

affinity chromatography: suitable

matrix active group

phase

swelling

1 g swells to 9-11 mL

bead size

20-80 μm

application(s)

life science and biopharma

compatibility

Cytiva

General description

Sephadex G-50 Fine is a widely used gel filtration resin designed for desalting and buffer exchange of biomolecules with a molecular weight greater than 30,000. Its small bead size contributes to higher efficiency in these processes. Different types of Sephadex vary in terms of cross-linking, resulting in differences in swelling and molecular fractionation range. Sephadex G-50 is one of five G-types available, ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 comes in four different particle sizes, such as Coarse, Medium, Fine, and Superfine.
Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatography, and gel filtration chromatography. Sephadex is a beaded gel prepared by crosslinking dextran with epichlorohydrin.
Fractionation Range (MW)
Globular Proteins: 1,500 - 30,000
Dextrans: 500 - 10,000

Application

Sephadex® G-50 has been used:
  • to remove the non-entrapped carboxyfluorescein (CF) from the liposome suspension
  • in the purification of monoclonal antibody (mAb) humanized MN-14 by centrifuged size-exclusion chromatography
  • in desalting the recombinant enzymes eluted in the fraction five and six

Sephadex® G-50 is suitable for use in:

  • the separation of low and high molecular weight molecules
  • affinity chromatography, protein chromatography, and gel filtration chromatography

Features and Benefits

  • Desalts, removes contaminants, and transfers to a new buffer in one step.
  • Suitable for DNA purification from small molecules using gel filtration.
  • Features a small bead size, resulting in shorter diffusion distances.
  • Considered a classic gel filtration resin.

  • Desalting with Sephadex is considered superior to dialysis because it saves time, has a low dilution factor, and recovers high activity even with minute amounts of sample

Other Notes

G5080-100G′s updated product number is GE17-0042-01
G5080-500G′s updated product number is GE17-0042-02

Legal Information

Sephadex is a registered trademark of Cytiva

replaced by

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L M Weiner et al.
Journal of immunology (Baltimore, Md. : 1950), 151(5), 2877-2886 (1993-09-01)
Bispecific monoclonal antibodies (BsmAb) with specificity for tumor Ag and effector cell trigger molecules have been shown to redirect the cytotoxicity of several peripheral blood mononuclear cell populations against relevant tumor. The BsmAb, 2B1, binds to the extracellular domain of
L Zhang et al.
The EMBO journal, 14(2), 313-320 (1995-01-16)
Heme is a prosthetic group for numerous enzymes, cytochromes and globins, and it binds tightly, sometimes covalently, to these proteins. Interestingly, heme also potentiates binding of the yeast transcriptional activator HAP1 to DNA and inhibits mitochondrial import of the mammalian
Samuel Lopez-Nieves et al.
The New phytologist, 217(2), 896-908 (2017-10-11)
Diverse natural products are synthesized in plants by specialized metabolic enzymes, which are often lineage-specific and derived from gene duplication followed by functional divergence. However, little is known about the contribution of primary metabolism to the evolution of specialized metabolic
B P Brunk et al.
Genetics, 124(1), 145-156 (1990-01-01)
The isolation of gain of function mutations has allowed the identification of a number of genes which are important in the normal development of the organism. We report here the isolation and characterization of Aristapedioid, a gain of function mutation
Jennifer E Dawson et al.
Nature communications, 11(1), 3146-3146 (2020-06-21)
Phosphorylation of intrinsically disordered eIF4E binding proteins (4E-BPs) regulates cap-dependent translation by weakening their ability to compete with eIF4G for eIF4E binding within the translation initiation complex. We previously showed that phosphorylation of T37 and T46 in 4E-BP2 induces folding
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