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Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer

lyophilized powder

Synonym(s):

ATP Assay Dilution Buffer, Assay Mix Buffer, Assay Mix for ATP

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.84

form

lyophilized powder

technique(s)

activity assay: suitable

storage temp.

−20°C

General description

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer is a component of the ATP bioluminescent assay kit that may be employed for the quantitative bioluminescent determination of ATP in experimental samples.

Application

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer has been used to determine the level of adenosine kinase activity in isolated sections of mouse hippocampi. It has also been used to measure the ATP content in oocytes and harderian gland(HG) tissue homogenates.

Components

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer contains MgSO4, dithiothreitol (DTT), ethylene diamine tetraacetic acid (EDTA, bovine serum albumin (BSA), and tricine buffer salts.

Reconstitution

Reconstitute with 50 mL of sterile water.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Peter Stief et al.
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Hana M Russo et al.
Journal of immunology (Baltimore, Md. : 1950), 197(4), 1353-1367 (2016-07-08)
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Nicolette Gouder et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 24(3), 692-701 (2004-01-23)
Endogenous adenosine in the brain is thought to prevent the development and spread of seizures via a tonic anticonvulsant effect. Brain levels of adenosine are primarily regulated by the activity of adenosine kinase. To establish a link between adenosine kinase
Quynh Thu Nguyen et al.
Biology open, 5(11), 1743-1751 (2016-09-11)
Mammalian spermatozoa utilize ATP as the energy source for key functions on the route to fertilization. ATP and its precursor nucleotides ADP and AMP are regularly investigated in sperm physiology studies, mostly by bioluminescence assays. Assay results vary widely, mainly
Nobuhiro Moro et al.
Experimental and therapeutic medicine, 21(6), 575-575 (2021-04-15)
The aim of the current study was to determine effects of mild traumatic brain injury (TBI), with or without blockade of purinergic ATP Y1 (P2Y1) receptors or store-operated calcium channels, on extracellular levels of ATP, glutamate, glucose and lactate. Concentrations

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