DNTP100
Deoxynucleotide Set, 100 mM
Individual dNTPs for routine PCR; 0.25 mL each
Synonym(s):
DNTP Set for PCR
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About This Item
UNSPSC Code:
41106305
NACRES:
NA.52
Recommended Products
form
liquid
concentration
100 mM
color
colorless
shipped in
dry ice
storage temp.
−20°C
General description
Deoxynucleotide triphosphates (dNTPs) are organic molecules, which contains nucleobase bound to deoxyribose. This dNTPs set includes 0.25 mL each of 100mM aqueous solutions of four deoxyribonucleotides, deoxyribose adenosine triphosphate (dATP), deoxyribose cytidine triphosphate (dCTP), deoxyribose guanosine triphosphate (dGTP), and deoxyribose thymidine triphosphate (dTTP) in separate tubes for convenience and easy PCR reaction setup.
Application
Deoxynucleotide Set, 100 mM has been used as a component of the reaction mixture for polymerase chain reaction (PCR).It has also been used as a component of the reaction mixture for amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to genotype single-nucleotide polymorphisms (SNPs).
Deoxynucleotide Set, 100 mM has been used:
- to biotinylate λ-DNA for magnetic tweezers measurements
- in RAPD PCR
- in FISH probe generation and labeling
- in PCR amplification of genomic DNA
- in multiplex PCR
- in first-strand cDNA synthesis
- in TUNEL labeling
- in RT-PCR
- as a component of the reaction mixture for polymerase chain reaction (PCR)
Biochem/physiol Actions
Deoxynucleotide triphosphates (dNTPs) are substrates for DNA polymerizing enzymes. They act as building blocks of nucleic acid molecules and are acidic in nature. dNTPs are key components of PCR master mixes and are highly essential to amplify DNA. Optimal levels of dNTPs ensure proper chromosomal replication and in turn maintain genome stability.
Features and Benefits
- Provided as 4 separate tubes of dNTPs.
- Allows optimization of individual dNTP concentrations.
Packaging
0.25 mL each of 100 mM dATP, dCTP, dGTP and dTTP
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Attractive forces between cation condensed DNA double helices
Todd BA, et al.
Biophysical Journal, 94, 4775-4782 (2008)
Multiple locus VNTR fingerprinting (MLVF) of Streptococcus pyogenes
Obszanska K, et al.
Virulence, 3(6), 539-542 (2012)
Detection of Streptococcus pyogenes virulence factors by multiplex PCR
Borek AL, et al.
Virulence, 3(6), 529-533 (2012)
Expression of Mx gene in Cirrhinus mrigala (Hamilton, 1822) to OmpC protein of Aeromonas hydrophila and bacterial infection
Roy P, et al.
Applied Biochemistry and Biotechnology, 178, 640-653 (2016)
A critical balance: dNTPs and the maintenance of genome stability
<BIG><BIG>Pai CC and Kearsey SE</BIG></BIG>
Genes, 8, 57-57 (2017)
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