D5921
Dulbecco′s Modified Eagle′s Medium - low glucose
With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
DME, DMEM
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About This Item
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Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
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General description
Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.
Application
Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture:
- living colon cancer SW620 cells
- human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231
- mammal and canine derived adipose-derived mesenchymal stem cells
Reconstitution
Supplement with 0.584 g/L L-glutamine.
Other Notes
Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
also commonly purchased with this product
Product No.
Description
Pricing
supplement
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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