D4522
Deoxyribonucleic acid from calf thymus
Type XV, Activated, lyophilized powder
Synonym(s):
Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52
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General description
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Features and Benefits
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• High quality template DNA
Quantity
One mg DNA is approx. 20 A260 units.
Preparation Note
Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.
Reconstitution
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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