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biological source
feline mouth
growth mode
Adherent
karyotype
2n = 38
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Cat tongue
Cell Line Description
Established from normal embryonal tongue tissue of female cat (Felis catus). Cells support the replication of a wide range of feline viruses. Can undergo 50 population doublings.
Application
Virus replication studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2 37°C. Maintain at pH 7.1-7.3.
Other Notes
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Certificates of Analysis (COA)
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