Sf9 Cell Line from Spodoptera frugiperda pupal ovarian tissue
89070101
Synonym(s):
SF-9 Cells
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About This Item
UNSPSC Code:
41106514
biological source
insect ovary
growth mode
Adherent
karyotype
Not specified
morphology
Spherical
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Spodoptera frugiperda pupal ovarian tissue
Cell Line Description
Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems.
Application
Sf9 cell line has been used for Baculovirus viral studies. It has also been used for in vitro kynurenine-3-monooxygenase (KMO) inhibition assay.
Culture Medium
TC100 + 2mM Glutamine. (The medium can be supplemented with 10% Foetal Bovine Serum (FBS) if required).
Subculture Routine
Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 9% DMSO/91% FBS. Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.
Other Notes
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Certificates of Analysis (COA)
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