CRI-D11

88031604

• UNSPSC Code: 41106514

Properties

• biological source: rat pancreas
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Insulin and glucagon
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat NEDH islet tumour

Cell Line Description

Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon.

Application

Hormone studies

Culture Medium

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cultures form monolayers of elongate cells.

Other Notes

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