CL 21
86080602, Human x Mouse Hybridoma unknown/unspecified, Fibroblast-like
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About This Item
UNSPSC Code:
41106514
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product name
CL 21, 86080602
biological source
Human x Mouse Hybridoma unknown/unspecified
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse / Human hybrid, SV-40 transformed
Cell Line Description
The cell line CL21 has been established by hybridisation of C57 BL mouse peritoneal macrophages with SV40 transformed human skin fibroblasts. The human cells were derived from a patient with Lesch-Nyhan syndrome and are deficient in hypoxanthine guanine phosphoribosyltransferase (HGPRT). They have been reported to contain the human chromosome 7 and could also contain subchromosomal fragments of chromosome 17.
Application
Somatic cell hybrid mapping
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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