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Key Documents

AFRC MAC 14

86030303, Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii

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About This Item

UNSPSC Code:
41106514

biological source

Rat x Rat Hybridoma

ImmunoDon

AO rat spleen

Myeloma

Y3Ag1.2.3

Reactivity

Anti-Glycoprotein of Chlamydomonas reinhardii

technique(s)

cell culture | mammalian: suitable

isotype

IgM

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat Y3Ag1.2.3 x Rat AO spleen

Cell Line Description

The antibody recognises the glycoprotein 2BII. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. AFRC MAC 14 shows similar fine specificity and properties to AFRC MAC 12, 13, 15, 16 and 17.

Immunogen

2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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