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SP2/O-Ag14 Cell Line murine

85072401, Non-producing hybridoma

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About This Item

UNSPSC Code:
12352207

biological source

mouse spleen

growth mode

Suspension

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mouse x Mouse myeloma, non-producing

Cell Line Description

Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. HAT sensitive mouse myeloma hybrid suitable for use as a hybridoma fusion partner - in this sense, functionally it is a myeloma cell line and often referred to as such in the literature.

Application

Fusion partner

Culture Medium

RPMI 1640 or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. HAT sensitive.

Other Notes

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Certificates of Analysis (COA)

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Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

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