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C7867

Sigma-Aldrich

Anti-Claspin (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
MFCD06411798
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 250 kDa (170 kDa band that may correspond to a claspin related protein is observed in some cell lines)

species reactivity

human

technique(s)

microarray: suitable
western blot: 1:250-1:500 using K562 cell extracts

UniProt accession no.

Q9HAW4

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CLSPN(63967)

Immunogen

synthetic peptide corresponding to amino acids 1321-1339 of human claspin, conjugated to KLH via an N-terminal added cysteine residue.

Application

Anti-Claspin (C-terminal) antibody is suitable for microarray and western blot analysis at a dilution of 1:250-1:500.

Biochem/physiol Actions

Claspin is a protein which plays a major role in DNA replication. It involves in protecting DNA replication forks against DNA damage. In DNA damage response, claspin directly interacts with Rad17, which is required for ATR activation. ATR is activated by various DNA damage and DNA replication stress. Once activated, ATR (ataxia telangiectasia mutated-Rad3 related protein) phosphorylates effector kinase Chk1 which further depends on mediator protein claspin. Claspin helps to stimulate phosphorylation reaction of Chk1.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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A Kumagai et al.
Molecular cell, 6(4), 839-849 (2000-11-25)
We have identified Claspin, a novel protein that binds to Xenopus Chk1 (Xchk1). Binding of Claspin to Xchk1 is highly elevated in the presence of DNA templates that trigger a checkpoint arrest of the cell cycle in Xenopus egg extracts.
Shizhou Liu et al.
Molecular and cellular biology, 26(16), 6056-6064 (2006-08-02)
TopBP1 and Claspin are adaptor proteins that facilitate phosphorylation of Chk1 by the ATR kinase in response to genotoxic stress. Despite their established requirement for Chk1 activation, the exact way in which TopBP1 and Claspin control Chk1 phosphorylation remains unclear.
Shizhou Liu et al.
Cell cycle (Georgetown, Tex.), 11(14), 2711-2716 (2012-06-27)
Claspin is a key mediator of the ATR-Chk1 checkpoint pathway. In response to DNA damage, Claspin interacts with Rad17 and Chk1 in a phosphorylation-dependent manner, enabling ATR to phosphorylate Chk1 efficiently. Claspin also interacts with Rad9, but how they interact
Yasunori Fukumoto et al.
The Journal of biological chemistry, 289(18), 12313-12329 (2014-03-19)
The DNA damage checkpoint arrests cell cycle progression to allow time for repair. Once DNA repair is completed, checkpoint signaling is terminated. Currently little is known about the mechanism by which checkpoint signaling is terminated, and the disappearance of DNA

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